ipure kit v2 (DIAGENODE DIAGNOSTICS)

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DIAGENODE DIAGNOSTICS ipure kit v2
Ipure Kit V2, supplied by DIAGENODE DIAGNOSTICS, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ipure+kit+v2/microplex+library+preparation+kit+v2/pm40563675-74-8-11
Average 90 stars, based on 1 article reviews

ipure kit v2 - by Bioz Stars, 2026-11

90/100 stars

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Incubation:

Article Title: Coupling Immunoprecipitation with Multiplexed Digital PCR for Cell-Free DNA Methylation Detection in Small Plasma Volumes of Early-Onset Colorectal Cancer
Article Snippet: .. Both IP and IC samples were incubated at 4 °C for 17 h before being purified using the IPure Kit v2 (Diagenode, Belgium) and eluted twice in 25 μL of buffer C to yield a final eluate of 50 μL. ..

Article Title: Coupling Immunoprecipitation with Multiplexed Digital PCR for Cell-Free DNA Methylation Detection in Small Plasma Volumes of Early-Onset Colorectal Cancer.
Article Snippet: .. Both IP and IC samples were incubated at 4 °C for 17 h before being puri ed using the IPure Kit v2 (Diagenode, Belgium) and eluted twice in 25 μL of buffer C to yield a nal eluate of 50 μL. ..

Purification:

Article Title: Genome-Wide cfDNA Methylation Profiling Reveals Robust Hypermethylation Signatures in Ovarian Cancer.
Article Snippet: Following adapter ligation, an immunoprecipitation step was performed to selectively enrich for methylated cfDNA using a 5-methylcytosine antibody (Clone 33D3, Diagenode, Liège, Belgium). .. The enriched methylated cfDNA underwent purification with the IPure Kit V2 (Diagenode) to remove contaminants and unbound DNA fragments. .. Library amplification was conducted with KAPA HiFi Hotstart ReadyMix (Roche) and Illumina P5 and P7 primers (IDT, primer sequences available in Table S2), with subsequent clean-up with 1.0× AMPure XP beads (Beckman Coulter, Brea, CA, USA).

Article Title: Tracking the genome-wide occupancy of Arabidopsis LEAFY COTYLEDON1 in endosperm development.
Article Snippet: The sonicated chromatin solution was diluted with ChIP dilution buffer and was further split into the necessary number of portions.Thealiquotwas incubatedwith anti-GFP (1:100,v:v) (CAT#ab290, Abcam, Cambridge, UK) overnight at 4 °Cwith gentle rotation, followed by incubation with Dynabeads protein A (Invitrogen, Waltham, MA) for 2 h. The beads were collected with amagnetic rack and washed by low salt wash buffer, high salt wash buffer and TE buffer. .. ChIP-DNA was purified from the washed beads by using a Ipure Kit v2 (Diagenode, NJ). ..

Article Title: Kinetochore mutations and histone phosphorylation pattern changes preceded holo- and macro-monocentromere evolution
Article Snippet: The mixed chromatins were incubated with the ClCENH3 antibody (10 mg/ml) to a final 1:500 dilution at 4°C by shaking at 14 rpm for 12 h. Dynabeads TM Protein A (Invitrogen) in ChIP dilution buffer, corresponding to 0.1× volume of the chromatin solution, was added to the antibody-prebound chromatins and incubated at 4°C by shaking at 14 rpm for 1.5 h. The collected beads were then washed twice in low salt buffer (150 mM NaCl, 0.1% SDS, 1% Triton X-100, 2 mM EDTA, 20 mM Tris-HCl pH 8.0), followed by three washes in high salt buffer (500 mM NaCl, 0.1% SDS, 1% Triton X-100, 2 mM EDTA, 20 mM Tris-HCl pH 8.0), and another two washes in TE buffer at 4°C by shaking at 14 rpm for 5 min. .. The bead-bound chromatin was purified by using iPure kit v2 (Diagenode) following the manual and quantified using Qubit TM dsDNA HS Assay kit (Invitrogen). .. ChIPseq libraries were prepared by NEBNEXT ® Ultra TM II DNA Library Prep Kit for Illumina (New England Biolabs) and sequenced using NovaSeq 6000 system (Illumina) by Novogene (UK) in the paired-end run (2×150 bp).

Article Title: Chromatin environment-dependent effects of DOT1L on gene expression in male germ cells
Article Snippet: .. ChIP was performed using iDeal ChIP-seq kit for transcription factors (Diagenode C01010055), and resulting DNA was purified using IPure Kit v2 (Diagenode C03010015) following the manufacturer’s recommendations. .. Anti-H3K79me2 antibody (Diagenode C15410051) was used in a ratio of 1 μg antibody per 5M cells.

Methylation:

Chromatin Immunoprecipitation:

Isolation:

Article Title: Coordinated improvement of maize grain yield and protein quality by the ZmMADS8-ZmMADS47-O2 module and a G protein gamma subunit
Article Snippet: Immunoprecipitation was performed with 1:3000 anti-GFP antibody (A11122, Thermo Fisher Scientific, Waltham, MA, USA) at 4 °C. .. DNA elution, decrosslinking and isolation was performed using an IPure Kit v2 according to the manufacturer’s instructions (C01010150, Diagenode SA, Seraing, Belgium). ..

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